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Figure 9 shows a ninth example of the three-dimensional microfluidic system according to the present invention.

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Figure 10 shows a tenth example of the three-dimensional microfluidic system according to the present invention. Figure 1 1 shows an eleventh example of the three-dimensional microfluidic system according to the present invention. Figure 12 shows a twelfth example of the three-dimensional microfluidic system according to the present invention. Figure 13 shows a thirteenth example of the three-dimensional microfluidic system according to the present invention. Figure 14 shows a fourteenth example of the three-dimensional microfluidic system according to the present invention.

Figure 15 shows a fifteenth example of the three-dimensional microfluidic system according to the present invention. Figure 16 shows a sixteenth example of the three-dimensional microfluidic system according to the present invention. Thread conducts the flow of liquids via capillary action, with the space between the cellulose fibres of the thread forming capillary channels. Blockage of these channels with glues or waxes can be used strategically to disable the capillary flow of liquids past a certain point in a thread.

Using this effect it is possible to simply create flow control devices with thread. Different reagents in multi-step reactions can be introduced into reaction zones simultaneously or separately by simply activating or deactivating switches. Thread-based devices can also be built as selectors which allow users to direct the samples or reagents they desire to a required location.

Moreover, thread can be used as a controllable mixer which is useful when a requirement to mix samples and reagents together at a specific time exists. All of these new and simple types of thread-based microfluidic device components are very low cost and can be easily fabricated without special laboratory equipment, and are therefore suitable for use in underdeveloped areas, remote regions or potentially as point-of-care products globally.

Thread channels can be blocked using commercially available cyanoacrylate-based fast acting adhesive to selectively inhibit liquid flow along a thread. With the application of adhesive or glue, no fluid penetration past the glue-sealed segment is possible. The glue completely blocks the inter-fibre channels within the thread.

Also, the glue can be employed to firmly fix thread onto polymer film. These blocking and adhesive characteristics allow switches to be built into thread-based microfluidic devices.

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Polypropylene polymer films can be used to support threads and fabricate fold, wedge and pull tab type switches all of which are detailed below. The following examples illustrate the operation of the three dimensional microfluidic system in a variety of different situations. The initial five examples illustrate the principles of operation of the three-dimensional microfluidic systems according to the present invention.

The remaining examples illustrate embodiments of the invention having flow control arrangements. Example 1. In one embodiment of the invention as shown in Figure 1 , a thread 3 of cotton fibre was treated with a plasma KX plasma asher Quorum Emitech, UK for 5 - 50 seconds at the intensity of 10 - 50 W to increase its hydrophilicity.


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The treated thread 3 showed no visible mark or colour change. Several pieces of treated thread 3 were then sewn through a polymer film 5 in a pattern shown in Figure 1 where there are several overlapping circles. This is a three-dimensional pattern where several pieces of thread cross one another, their stitches passing each other from above and below the polymer film; they therefore do not have contact. These threads define the capillary passageways without the need of a barrier.

When fluids were introduced on to different threads, they travel along the thread by capillary effect. Because of the three-dimensional structure, the microfluidic system device allowed the fluids to travel independently without mixing. Such a microfluidic system can be built in multilayers to form a complex fluid transport device. Such a microfluidic system can be used to transport a wide range of fluids, including hydrocarbons, which cannot be transported by microfluidic systems with PDMS barriers.

Example 2. The second embodiment of the invention as shown in Figure 2, illustrates how a hydrophilic thread can transport a fluid sample without being woven or sewn into a supporting substrate. When one hydrophilic thread 6 forms a braid with a hydrophobic thread 7, the fluid only travels along the hydrophilic thread 6. Figure 2 shows a braid of two cotton threads with one treated with plasma 6 and the other not treated 7.

Hydrophilic characteristics of the thread are provided by the plasma treatment. Fluid introduced onto the treated thread 6, only travelled along that hydrophilic thread 6. This has a practical benefit in that the present invention can be incorporated within textiles used for clothing as well as other applications. When using a sewing machine to sew thread through textile, some machines use two threads, one which goes with the needle, and the other which comes from the bottom. If one of these threads is hydrophilic and the other hydrophobic, liquid will only transport through the hydrophilic thread.

Or, a pattern can be sewn where one thread hydrophobic holds the other hydrophilic , but with only hydrophilic thread transporting fluid.

The hydrophobic thread that holds the hydrophilic thread will not transport fluid. Alternatively, a hydrophilic thread in a rope can be used to transport or detect a fluid sample, but other threads in the rope will merely be used to provide strength, not transporting fluid samples. Example 3. The third embodiment of the invention as shown in Figure 3, illustrates how a microfluidic system according to the present invention can be made using hydrophilic threads to mix different fluids during fluid transport along the threads. Figure 3 shows, on the left, three plasma treated threads B, Y, M taking fluids of three different colours; with the uppermost thread transporting a blue fluid B, the middle thread transporting a yellow fluid Y, and the lowermost thread transporting a magenta fluid M.

The uppermost and middle threads were braided at a central mixing area C whereas the lowermost thread is not. The fluids of the two braided threads B, Y mixed to form green fluid G to the right of the central mixing area C, whereas the magenta fluid transported in the lowermost thread M did not undergo mixing as it was not braided with the other threads. The fourth embodiment of the invention as shown in Figure 4, illustrates how a hydrophilic thread can be used to make a sample detector.

The amount in volume of the liquid introduced onto a thread results in a length of wicking that is proportional to the volume added. The colour change on a single stitch of thread due to the passage of fluid along its length is sufficient to deliver visual detection. Figure 4 shows a series of hydrophilic threads where 0. The lengths of the thread that have changed colour due to the transportation of the fluid along the thread are almost proportional to the fluid volumes introduced.

The point where the fluid was introduced on each thread is labelled 10 in Figure 4. The uppermost thread had 0.

The fluid travelled to the point marked 1 1 in Figure 4. The point marked 12 corresponds to the point where 0.

Similarly, point 13 indicates where 0. This proportionality means that the colour density of the liquid on the thread is not affected by how much volume of each sample is added onto a thread. The colour density of the stain will be the same, since the amount of dye per unit length of the stained thread is the same. Therefore even without an accurate liquid handling device, good analytical results can be obtained. The fifth embodiment of the invention as shown in Figure 5, illustrates how a hydrophilic thread can be used to construct microfluidic sensors with other materials including, but not restricted by, paper, textile and other woven, non- woven material, powdery, gel, wax and a wide range of other materials.

In Figure 5 there is shown fluid transported through an aluminium foil substrate 20, with a piece of paper 21 being used as a detector to show the arrival of the fluid.